Identification of Gating Mutations in the Trek-1 k2p Potassium Channel by Functional Complementation in K+ uptake Deficient Yeast
Biophysical Journal Elsevier 100:3 (2011) 279a-280a
PIP2-Binding to an Open State Model of Kir1.1 Probed by Multiscale Biomolecular Simulations
Biophysical Journal Elsevier 100:3 (2011) 431a
The Kir5.1 Potassium Channel is an Important Determinant of Neuronal PCO2/pH Sensitivity
Biophysical Journal Elsevier 100:3 (2011) 430a
Genetic inactivation of Kcnj16 identifies Kir5.1 as an important determinant of neuronal PCO2/pH sensitivity.
The Journal of biological chemistry 286:1 (2011) 192-198
Abstract:
The molecular identity of ion channels which confer PCO(2)/pH sensitivity in the brain is unclear. Heteromeric Kir4.1/Kir5.1 channels are highly sensitive to inhibition by intracellular pH and are widely expressed in several brainstem nuclei involved in cardiorespiratory control, including the locus coeruleus. This has therefore led to a proposed role for these channels in neuronal CO(2) chemosensitivity. To examine this, we generated mutant mice lacking the Kir5.1 (Kcnj16) gene. We show that although locus coeruleus neurons from Kcnj16((+/+)) mice rapidly respond to cytoplasmic alkalinization and acidification, those from Kcnj16((-/-)) mice display a dramatically reduced and delayed response. These results identify Kir5.1 as an important determinant of PCO(2)/pH sensitivity in locus coeruleus neurons and suggest that Kir5.1 may be involved in the response to hypercapnic acidosis.Genetic Inactivation of Kcnj16 Identifies Kir5.1 as an Important Determinant of Neuronal PCO2/pH Sensitivity
JOURNAL OF BIOLOGICAL CHEMISTRY 286:1 (2011) 192-198