Gene machines

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Rapid functionalisation and detection of viruses via a novel Ca2+-mediated virus-DNA interaction

Scientific Reports Nature Research 9 (2019) 16219

Authors:

Nicole Robb, Jonathan Taylor, Amy Kent, A Kapanidis, O Pambos, B Gilboa

Confinement-free wide-field ratiometric tracking of single fluorescent molecules

Biophysical Journal Elsevier 117:11 (2019) 2141-2153

Authors:

Barak Gilboa, B Jing, TJ Cui, M Sow, A Plochowietz, A Mazumder, AN Kapanidis

High-Throughput Detection and Manipulation of Single Nitrogen-Vacancy Center's Charge in Nanodiamonds

(2019)

Authors:

Maabur Sow, Horst Steuer, Sanmi Adekanye, Laia Gin茅s, Soumen Mandal, Barak Gilboa, Oliver A Williams, Jason M Smith, Achillefs N Kapanidis

Recent Advances in Understanding 蟽70-Dependent Transcription Initiation Mechanisms.

Journal of molecular biology (2019)

Authors:

Abhishek Mazumder, Achillefs N Kapanidis

Abstract:

Prokaryotic transcription is one of the most studied biological systems, with relevance to many fields including the development and use of antibiotics, the construction of synthetic gene networks, and the development of many cutting-edge methodologies. Here, we discuss recent structural, biochemical, and single-molecule biophysical studies targeting the mechanisms of transcription initiation in bacteria, including the formation of the open complex, the reaction of initial transcription, and the promoter escape step that leads to elongation. We specifically focus on the mechanisms employed by the RNA polymerase holoenzyme with the housekeeping sigma factor 蟽70. The recent progress provides answers to long-held questions, identifies intriguing new behaviours, and opens up fresh questions for the field of transcription.

Recent advances in understanding 蟽70-dependent transcription initiation mechanisms

Journal of Molecular Biology Elsevier 431:20 (2019) 3947-3959

Authors:

Abhishek Mazumder, Achillefs Kapanidis

Abstract:

Prokaryotic transcription is one of the most studied biological systems, with relevance to many fields including the development and use of antibiotics, the construction of synthetic gene networks, and the development of many cutting-edge methodologies. Here, we discuss recent structural, biochemical, and single-molecule biophysical studies targeting the mechanisms of transcription initiation in bacteria, including the formation of the open complex, the reaction of initial transcription, and the promoter escape step that leads to elongation. We specifically focus on the mechanisms employed by the RNA polymerase holoenzyme with the housekeeping sigma factor 蟽70. The recent progress provides answers to long-held questions, identifies intriguing new behaviours, and opens up fresh questions for the field of transcription.